Entry 1 sense Webster, 1913 Sporulation /(spŏr`ū̇*lā"shŭn)/ · Spor·u·la·tion · IPA 01 n. The act or process of forming spores; spore formation. See Illust. of Bacillus, (b). 1. The act or process of forming spores; spore formation. See Illust. of Bacillus, (b).(Biol.) See: Bacillus